Plasmid

Part:BBa_J100310

Designed by: Zachary Shaver   Group: Campbell M Lab   (2016-11-14)


J100287 with two bases removed between gene III and GFP

J100287 is a plasmid that could be used in the positive selection phase of Phage Assisted Continuous Evolution (PACE) to evolve riboswitches. Gene III, the gene necessary for phage survival, and GFP, a reporter gene, are separated by a stop codon at the end of gene III. J100310 had two bases, specifically "TA", in the stop codon between the genes removed, forming a translational fusion of the two proteins. While both gene III and GFP should be produced in J100287 since the GFP gene has its own ribosome binding site, J100310 is another option to try for PACE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4048


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